Research Article
Tumor-Targeted Folic Acid-Modified Porfimer Conjugated Poly (Ethylene Glycol) Nanoparticles for Radiosensitized Treatment of Cancer
Song-Jin Pak,
Chang-Jun Kim,
Kwang-Il To*
,
Nam-Hyok Ooh,
Mun-Hyok Ri,
Chol-Ho Ri,
Nam-Yong Kim
Issue:
Volume 10, Issue 2, June 2025
Pages:
20-28
Received:
7 April 2025
Accepted:
29 April 2025
Published:
12 June 2025
DOI:
10.11648/j.bmb.20251002.11
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Abstract: Folic acid-modified porfimer conjugated poly (ethylene glycol) nanoparticles (FPP NPs), as the novel radiosensitizer, for tumor-targeted radiosensitized treatment (RST) were synthesized and characterized. Diamino poly (ethylene glycol) was covalently conjugated with folic acid and porfimer (also known as photofrin) in its terminal ends and self-assembled in aqueous solution to form FPP NPs. FPP NPs were characterized by FTIR spectrometer, zetasizer and transmission electron microscopy. FT-IR spectra of folic acid-modified porfimer conjugated poly (ethylene glycol) suggested that folic acid and porfimer were successfully covalently bound to poly (ethylene glycol) by amide bonds between carboxyl group and amino group. The average size and zeta potential of these nanoparticles were about 75nm and -13.5mV, respectively. And FPP NPs exhibited morphological features close to spherical shape by transmission electron microscopy observation. The loading efficiency and encapsulation efficiency of porfimer in FPP NPs were 8.52% and 45.67%, respectively. The experiments on reactive oxygen species (ROS) generation and cell viability showed that the nanoparticles could successfully suppress the growth of cancer cells by producing ROS with γ-ray irradiation. Furthermore, in vivo studies showed that FPP NPs exhibited the excellent tumor inhibition effect even at a half dose of free porfimer. These results suggested that FPP NPs would have potential for RST of the cancer.
Abstract: Folic acid-modified porfimer conjugated poly (ethylene glycol) nanoparticles (FPP NPs), as the novel radiosensitizer, for tumor-targeted radiosensitized treatment (RST) were synthesized and characterized. Diamino poly (ethylene glycol) was covalently conjugated with folic acid and porfimer (also known as photofrin) in its terminal ends and self-ass...
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Research Article
Release and Registration of “Hundaol” Intermediate Maturing Type of Cowpea (Vigna Unguiculata (L.) Variety for Mid and Lowland of West Hararghe, Oromia, Ethiopia
Tamrat Dinkale*,
Muleta Debela,
Birmaduma Gadisa,
Jibrail Hassan,
Lensa Urgesa
Issue:
Volume 10, Issue 2, June 2025
Pages:
29-36
Received:
25 February 2025
Accepted:
28 March 2025
Published:
26 June 2025
DOI:
10.11648/j.bmb.20251002.12
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Abstract: Hundaol” is a name given for the cowpea variety with accession number ILRI#25368 developed and released variety for dual-purpose (grain seed and forage) production for low and mid-altitude areas of West Hararghe. “Hundaol” was tested at Mechara Agricultural Research Center on station, Milkaye farmer training center, and Melkasa Agricultural Research Center Mieso sub-site for two years from 2019- 2021 with nine other genotypes and two standard checks. Hundaol variety was sown with a seed rate of 30kg/ha, 100 kg/ha NPSB, and 50 kg/ha urea fertilizer rate, which was applied at a sowing time with a space of 30 cm between rows. “Hundaol” was selected for its best seed yield, highest dry matter yield, disease and pest tolerance, and stable performance across the locations. “Hundaol” was verified at on station and the farmer’s field level during the variety verification trial in 2023 with the newly released standard check “Qophee” variety. “Hundaol” produced 9.68 t/ha dry matter yields that have yield advantages of 12.30% and 89.25% over White Wonder and Kenketi standard checks, respectively. It produce an average seed yield of 23.28 qt/ha. On average, “Hundaol” variety has 118.86 cm plant height, 15.3cm, 13.2, and 12.90 pod length, number of pods per plant and seed per pod respectively, needs 67.61 days to reach 50% of flowering, 108.67 days to reach seed maturity. Similarly, the experimental farmers gave the first rank for the “Hundaol” variety rather than the standard check “Qophee” variety. So, the introduction of these newly released forage varieties could help alleviate the feed by small-scale farmers in West Hararghe and other parts of the country.
Abstract: Hundaol” is a name given for the cowpea variety with accession number ILRI#25368 developed and released variety for dual-purpose (grain seed and forage) production for low and mid-altitude areas of West Hararghe. “Hundaol” was tested at Mechara Agricultural Research Center on station, Milkaye farmer training center, and Melkasa Agricultural Researc...
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